NettetThere appeared to be two distinct types of cell death, resulting from the use of either free PEI (which acts within 2 h) or PEI/DNA complexes (which cause death 7-9 h after … Nettet3. apr. 2024 · Suspension cell lines have traditionally proven to be very difficult to transfect. The common reason for this is a reduced attachment of the transfection complex, a mixture of the gene of interest with a mostly liposome-based transfection reagent, to the surface of the cells that results in a reduced uptake of the target DNA ( …

Optimizing the transient transfection process of HEK-293 ... - PubMed

NettetIn contrast, polyethylenimine (PEI) is much easier to use: It binds to and precipitates DNA efficiently and the resulting DNA-PEI complexes are suitable for efficient transfection of mammalian cells. PEI has been used successfully on a large scale in serum-containing and serum-free cultures. NettetPEI is a polymer-based reagent, sort of taking inspiration from lipofection approaches, which can be more custom-oriented for given cell lines (e.g. HeLa: … intern learning contract

Plasmid DNA Transfection Protocol - YouTube

NettetThe increase in transfection efficiency was obtained when the buffering capacity of the PEI/DNA was increased without a change in surface charge concentration, which … NettetAs with most transfection reagents, PEI-mediated transfection involves the formation of nanoparticles (polyplexes) which are obtained by its mixing with plasmid DNA. A short incubation period that allows polyplexes to reach their optimal size is performed prior to their addition to the culture. intern learning plan