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A custom insert was constructed as follows. Two BRAND Inserts for 12-well plates PET-membrane 0.4 µm (BrandTech Scientific Inc., CAT# 782730, Essex, CT, USA) were used to construct a “3D-Flipwell” (Figs. 2, 3). All work was performed under sterile conditions inside a cell culture cabinet (Class II A/B3 Biological … See more Caco-2 human colon epithelial cells were obtained from American Tissue Culture Collection (ATCC, CAT#: HTB-37, Manassas, VA). THP … See more The following reagents were used. l-Sepiapterin (SEP, Career Henan Chemical Co., CAS#: 17094-01-8, National University Science Park, High-Tech Zone, Zhengzhou City, … See more THP-1 cells were maintained at a cell density of 1 × 105/mL to 1 × 106/mL in RPMI 1640 (ThermoFisher Scientific, CAT#: 11875119, Waltham, MA) supplemented with 10% FBS, 1% GlutaMax … See more The following antibodies were used. Anti-human MUC2 (996/1) (ThermoFisher, CAT# MA5-12345); Anti-human CD68 (Novus Biologicals, CAT# NB100-683-0.1 mg, Centennial, CO, USA); Anti-human CK20 (Life … See more WebFlipWell is a real estate company with 1 Ci User. This company has been part of Connected Investors since 06/29/2024 - FlipWell is interested in CHESTERTOWN, MD. Connect … notts v derby cricket
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